Azolla filiculoides Lamarck

Azolla can be placed in its own family, Azollaceae, but is often included with Salvinia in the family Salviniaceae. Salviniaceae and Marsileaceae (Marsilea, Pilularia and Regnellidium) form a monophyletic group of water ferns, which are all heterosporous, ie. produce two kinds of spores, large female megaspores and small male microspores.Many fossil species of Azolla are known from the Upper Cretaceous. Today there are only 6 or 7 species in this genus.Azolla species are difficult to identify because high magnification is required to see the distinguishing characters clearly. In addition, the plants are often sterile and thus lack most of the features essential for identification. This has led to many misidentifications, confused taxonomy and thus uncertainty over species distributions.

Morphology
The small plants of Azolla filiculoides are prostrate, with branching thread-like horizontal stems generally up to 5cm long.Numerous unbranched roots up to 5cm long hang below the plant, arising from the underside of the stem at branching points.The tiny leaves (no more than 1.5 x 2.5mm in size) are alternately arranged in 2 rows on the upper side of the stem and overlap like roof tiles.Each leaf is divided into 2 lobes:

• the upper lobe is green, several cells thick and bears numerous single-celled papillate hairs
• the upper lobe has a cavity in which the thread-like blue-green alga (cyanobacterium) Anabaena azollae lives in symbiosis
• the lower lobe is more-or-less colourless and only one cell thick except at the base

Spores are produced inside sporocarps. The sporocarps are borne in pairs (sexes the same or different) on the first leaf of each branch, the lower lobe forming the sporocarp and the upper lobe forming a false indusium over the sporocarp:

• megasporocarps (female; about 0.5mm diameter) contain just one megasporangium, inside which a single megaspore develops
• as the megaspore matures, floats develop above it, topped with a colony of cyanobacteria and covered by a dark conical indusium; collectively this is known as the ‘megaspore apparatus’
• microsporocarps (male; about 2mm diameter) contain numerous sporangia, each containing 64 microspores
• the microspores are clumped together with other tissue in massulae that bear barbed hairs called glochidia

Look-alikes
Azolla caroliniana and A. mexicana are very similar to A. filiculoides. Although A. filiculoides is the common species in western Europe, both the others have been recorded, though some of the identifications are questionable. All three species are native in the Americas.They differ principally in:

• the number of cells in the hairs on the upper leaf lobes - A. filiculoides has single-celled hairs whereas those of A. caroliniana and A. mexicana have at least 2 cells (light microscope required)
• the nature of the surface of the megaspore (female spore) – A. filiculoides has megaspores with a warty surface, while those of A. caroliniana and A. mexicana are not warty (scanning electron microscope needed)

Have you recently seen a pond, lake or ditch that looks pink or red? If so, you have almost certainly seen Azolla.Azolla species are the world’s smallest, but most economically important ferns.In many ways Azolla is a very atypical fern as it:

• is aquatic, floating on the surface of ponds and lakes, ditches and canals
• looks more like a leafy liverwort than a fern, having minute overlapping leaves
• propagates itself readily by breaking up to form separate plants
• reproduces by means of two kinds of very unusual spores
• often changes colour dramatically in autumn or winter - turning from green to a distinctive deep red colour
• contains a blue-green alga (cyanobacterium) called Anabaena azollae that fixes atmospheric nitrogen

The presence of the nitrogen-fixing cyanobacterium has led to Azolla’s use as a green fertiliser, particularly in rice paddies.Unfortunately Azolla spreads so rapidly by vegetative propagation that it has become a widespread weed of water bodies in the UK and around the world. Biological control using a species of weevil is an alternative to using herbicides or physically removing the plants.

Perennial, mat-forming, free-floating aquatic. Rhizomes up to 25 - 35 mm long, branched, bearing roots singly or in clusters of 2-3. Leaves 2-lobed, each lobe 0.5-1.5 mm long, silvery-green, turning red in winter. Dorsal lobe broadly ovate to almost circular, apex rounded, margin translucent; ventral lobe like dorsal lobe but completely translucent. Plant heterosporous.

filiculoides: resembling a fern (from the Latin, filicum for a fern); unclear reference since Azolla does not coincide with the image that one has of a fern